Utilización de la PCR para el diagnóstico en Ictiopatología

Alicia Gibello, Maria del Mar Blanco, Lucas Domínguez, José F. Fernández- Garayzábal


Las enfermedades infecciosas constituyen uno de los principales problemas en piscicultura, tanto desde el punto de vista sanitario como económico. Realizar de forma rápida el diagnóstico de los animales enfermos y evitar el desarrollo de ciertas enfermedades mediante la detección de los animales portadores del patógeno constituyen, junto con la vacunación y las buenas prácticas de manejo, las medidas más eficaces de prevención de estas patologías. En las últimas décadas se han desarrollado diversas técnicas de diagnóstico rápido basadas
en métodos genéticos, entre los que destaca la reacción en cadena de la polimerasa (PCR). En este trabajo se evalúan las ventajas e inconvenientes de la utilización de la técnica de PCR en la detección de los microorganismos patógenos para los peces.


From a sanitary and an economical point of view, infectious diseases are one of the most important problems in pisciculture. The rapid establishment of the diagnosis of diseased animals, as well as the detection of carrier fish can provide a highly eficiency system to prevent these diseases. In the last decades, several diagnostic methods based on genetic technology have been developed, but PCR is more frecuently used than any other nucleic acid based techniques. The purpose of this review is to examinate the use of PCR as an approach to diagnosis of fish pathogens.

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